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Influence of Fe3O4 Nanoparticles in Hydroxyapatite Scaffolds on Proliferation of Primary Human Fibroblast Cells

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Abstract

Modern techniques for expanding stem cells play a substantial role in tissue engineering: the raw material that facilitates regeneration of damaged tissues and treats diseases. The environmental conditions and bioprocessing methods are the primary determinants of the rate of cultured stem cell proliferation. Bioceramic scaffolds made of calcium phosphate are effective substrates for optimal cell proliferation. The present study investigates the effects of two bioceramic scaffolds on proliferating cells in culture media. One scaffold was made of hydroxyapatite and the other was a mixture of hydroxyapatite and ferromagnetic material (Fe3O4 nanoparticles). Disk-shaped (10 mm × 2 mm) samples of the two scaffolds were prepared. Primary human fibroblast proliferation was 1.8- and 2.5-fold faster, respectively, when cultured in the presence of hydroxyapatite or ferrous nanoparticle/hydroxyapatite mixtures. Optical microscopy images revealed that the increased proliferation was due to enhanced cell-cell contact. The presence of magnetic Fe3O4 nanoparticles in the ceramic scaffolds significantly increased cell proliferation compared to hydroxyapatite scaffolds and tissue culture polystyrene.

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Authors and Affiliations

  1. Faculty of Materials Engineering, Sahand University of Technology, P.O. Box: 51335-11996, Tabriz, Iran

    H. Maleki-Ghaleh

  2. Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada

    E. Aghaie

  3. Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran

    A. Nadernezhad

  4. Faculty of Science and Engineering, Sharif University of Technology, International Campus, Kish, Iran

    M. Zargarzadeh

  5. Materials and Energy Research Center, Karaj, Iran

    A. Khakzad & M. S. Shakeri

  6. Chemical Engineering Group, Faculty of Engineering, Azarbaijan Shahid Madani University, Tabriz, Iran

    Y. Beygi Khosrowshahi

  7. Faculty of Materials Science and Engineering, K. N. Toosi University of Technology, Tehran, Iran

    M. H. Siadati

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  1. H. Maleki-Ghaleh
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Maleki-Ghaleh, H., Aghaie, E., Nadernezhad, A. et al. Influence of Fe3O4 Nanoparticles in Hydroxyapatite Scaffolds on Proliferation of Primary Human Fibroblast Cells. J. of Materi Eng and Perform 25, 2331–2339 (2016). https://doi.org/10.1007/s11665-016-2086-4

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  • DOI: https://doi.org/10.1007/s11665-016-2086-4

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